Journal: bioRxiv

Article Title: Mechanical, Biochemical, and Multicellular Effects on Vessel Network Morphometrics in a Microfluidic Vasculature-on-a-Chip

doi: 10.1101/2025.08.28.672831

Figure Lengend Snippet: a) Fluorescence images showing vessel network development over 7 days (images shown are for FB1:EC5). Red: RFP-HUVECs; green: GFP-normal human lung fibroblasts. b) Illustration of fluorescent microbeads perfused through MVN and sample fluorescence image of microbeads in MVN. c) Overlay fluorescence image of microbeads (blue) in the vessel network (red), with two insets. (i) Magnification of inset showing a vessel branch with no beads, suggesting perfusion did not reach that branch (white arrows). (ii) Magnification of inset with all vessel branches perfused by beads. Scale bar = 500 mm for a) to c). d) Beads leaked out of vessels frequently near branches with jagged edges. e) Vessel branches with smooth edges contained the beads without leakage. f) Localized vessel leak near a vessel opening. (Top: raw image of vessels; Bottom: red = vessels, green = beads).

Article Snippet: Red fluorescent protein-expressing human umbilical vein endothelial cells (RFP-HUVECs, cAP-0001RFP) and green fluorescent protein-expressing human adult lung fibroblasts (GFP-hLFs, cAP-0033GFP) were purchased from Angio-Proteomie (Boston, MA, USA) and cultured on T75 flasks coated with Quick Coating solution (cAP-01, Angio-Proteomie) according to manufacturer’s protocol.

Techniques: Fluorescence

Journal: bioRxiv

Article Title: Mechanical, Biochemical, and Multicellular Effects on Vessel Network Morphometrics in a Microfluidic Vasculature-on-a-Chip

doi: 10.1101/2025.08.28.672831

Figure Lengend Snippet: Morphometric analysis of fibroblast effects on MVN morphology: branched-based metrics. a) Fluorescence images of RFP-HUVECs forming MVNs in the middle channel. Scale bar = 500 mm. b) Line plots of average percent vessel area over time. Shaded regions represent standard deviations. c) Bar graph of average percent vessel area grouped by days in culture. Each data point represents percent vessel area of an individual device unit (n=10, 23, 26, 16, and 12 for EGM-2, FB1:EC5, FB5:EC5, FB-CM, and Ang-V50, respectively). d) Boxplot of individual vessel branch diameters (measurable, as defined in ) on Day 7. Red line is the median; box ranges from 25 th to 75 th percentiles. Each data point represents a single vessel diameter, collected from all the device units for each condition. e) Bar graph of number of diameter outliers (as defined in ) per device unit. For d) and e), n = 10, 23, 32, 16, and 12 for EGM-2, FB1:EC5, FB5:EC5, FB-CM, and Ang-V50, respectively. A few vessel units included in d) and e) were excluded from b) and c) because the prerequisite binary conversion using a global threshold failed to capture the true morphology of the vessel network in these units. For all graphs: *p<0.05, **p<0.01, ***p<0.001.

Article Snippet: Red fluorescent protein-expressing human umbilical vein endothelial cells (RFP-HUVECs, cAP-0001RFP) and green fluorescent protein-expressing human adult lung fibroblasts (GFP-hLFs, cAP-0033GFP) were purchased from Angio-Proteomie (Boston, MA, USA) and cultured on T75 flasks coated with Quick Coating solution (cAP-01, Angio-Proteomie) according to manufacturer’s protocol.

Techniques: Fluorescence

Journal: bioRxiv

Article Title: Mechanical, Biochemical, and Multicellular Effects on Vessel Network Morphometrics in a Microfluidic Vasculature-on-a-Chip

doi: 10.1101/2025.08.28.672831

Figure Lengend Snippet: Morphometric analysis of fibroblast effects on MVN morphology: void-based metrics. a) Temporal line plots and d) bar graph (grouped by days in culture) of void count per device unit. b) Temporal line plots and e) box plot (grouped by days in culture) of average void area per device unit. c) Temporal line plots and f) box plot (grouped by days in culture) of average void roundness per device unit. Shaded regions represent standard deviations. Each data point represents either total void count in a device unit for d), or the average void area or void roundness across all voids in a device unit for e) and f). g) Polar histograms of void angles over time. Angles measured from the horizontal x-axis as defined in . Angles between 90 to 180 degrees were converted to their supplementary angles. For a) to g): n = 10, 23, 26, 16, and 12 for EGM-2, FB1:EC5, FB5:EC5, FB-CM, and Ang-V50, respectively. h) Bar graph of percent perfusable vessel networks, calculated as number of perfusable MVN units divided by total tested MVN device units. Blue: perfusable units with leakage Red: perfusable units without leakage. For h): n = 10,18,29,12, and 12 for EGM-2, FB1:EC5, FB5:EC5, FB-CM, and Ang-V50, respectively. *p<0.05, **p<0.01, ***p<0.001.

Article Snippet: Red fluorescent protein-expressing human umbilical vein endothelial cells (RFP-HUVECs, cAP-0001RFP) and green fluorescent protein-expressing human adult lung fibroblasts (GFP-hLFs, cAP-0033GFP) were purchased from Angio-Proteomie (Boston, MA, USA) and cultured on T75 flasks coated with Quick Coating solution (cAP-01, Angio-Proteomie) according to manufacturer’s protocol.

Techniques:

Journal: Oncotarget

Article Title: Nicotinic acetylcholine receptors induce c-Kit ligand/Stem Cell Factor and promote stemness in an ARRB1/ β-arrestin-1 dependent manner in NSCLC

doi:

Figure Lengend Snippet: Microarray was performed in ARRB1 depleted and nicotine stimulated A549 cells Nicotine induced and ARRB1 dependent genes from the microarray data were analyzed. We identified differentially regulated genes that were regulated by nicotine in a β-arrestin-1 dependent fashion and top 10 up/down regulated genes from the list were used for prognosis prediction. Assessment of the expression of these genes for smoking revealed that SCF (highlighted in red) strongly differentiated smokers from non-smokers implying an important role of this gene in lung carcinogenesis induced by smoking

Article Snippet: A human lung cancer tissue microarray slide (Catalog number IMH-358; Imgenex, San Diego, CA) was immunostained for SCF.

Techniques: Microarray, Expressing, Control